![]() ![]() A customized donor bead, modified for excitation outside the range of photosystem absorbance, was tested for its capacity to improve assay sensitivity with extracts containing photosynthetic pigments. In assays containing photosynthetic matrices, it is proposed that excitation of chlorophyll leads to the production of singlet oxygen, which initiates a pigment-associated background signal, reducing assay sensitivity. However, its 680 nm excitation wavelength coincides with a wavelength of peak photosynthetic pigment absorbance, hindering the technology’s utility within the plant biotechnology industry. For the purposes of high-throughput immunoassay screening, PerkinElmer’s AlphaLISA technology offers many benefits over traditional enzyme-linked immunosorbant assay (ELISA).
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